LOCUS       plasmid                 7252 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             4130..4990
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        5964..6343
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        6344..6547
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   3073..3213
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    2454..2565
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4025..4129
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      5161..5749
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      3288..3743
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             6595..6621
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        2694..2934
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(3218..3269)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2370..2392
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(3744..3793)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3895..3930)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             2340..2362
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   5822..5849
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   5878..5905
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 ccctggaaca gatcggcggc aatagctgtg cctctggacc tgtgggaaaa gccctgagcg
       61 acgcctgtat gggagccgtg gcatccttcc tgaccaagta ccaggacatc atcctggaac
      121 accagaaagt gatcaagaag aacgagaaaa gactggccaa cctcaaggat atcgccagcg
      181 ctaacggcct ggcctttcct aagatcaccc tgcctccaca gcctcacacc aaagagggca
      241 tcgaggccta caacaacgtg gtggcccaga tcgtgatttg ggtcaacctg aatctgtggc
      301 agaagctgaa gatcggcagg gacgaagcca agccactgca gagactgaag ggcttcccta
      361 gcttccctct ggtggaaaga caggccaatg aagtggattg gtgggacatg gtctgcaacg
      421 tgaagaagct gatcaacgag aagaaagagg atggcaaggt tttctggcag aacctggccg
      481 gctacaagag acaagaagcc ctgctgcctt acctgagcag cgaagaggac cggaagaagg
      541 gcaagaagtt cgccagatac cagttcggcg acctgctgct gcacctggaa aagaagcacg
      601 gcgaggactg gggcaaagtg tacgatgagg cctgggagag aatcgacaag aaggtggaag
      661 gcctgagcaa gcacattaag ctggaagagg aaagaaggag cgaggacgcc caatctaaag
      721 ccgctctgac cgattggctg agagccaagg ccagctttgt gatcgagggc ctgaaagagg
      781 ccgacaagga cgagttctgc agatgcgagc tgaagctgca gaagtggtac ggcgatctga
      841 gaggcaagcc cttcgccatt gaggccgaga acagcatcct ggacatcagc ggcttcagca
      901 agcagtacaa ctgcgccttc atttggcaga aagacggcgt caagaaactg aacctgtacc
      961 tgatcatcaa ttacttcaaa ggcggcaagc tgcggttcaa gaagatcaaa cccgaggcct
     1021 tcgaggctaa cagattctac accgtgatca acaaaaagtc cggcgagatc gtgcccatgg
     1081 aagtgaactt caacttcgac gaccccaacc tgattatcct gcctctggcc ttcggcaaga
     1141 gacagggcag agagttcatc tggaacgatc tgctgagcct ggaaaccggc tctctgcgcc
     1201 tggccaatgg cagagtgatc gagaaaaccc tgtacaacag gagaaccaga caggacgagc
     1261 ctgctctgtt tgtggccctg accttcgaga gaagagaggt gctggacagc agcaacatca
     1321 agcccatgaa cctgatcggc atcgaccggg gcgagaatat ccctgctgtg atcgccctga
     1381 cagaccctga aggatgccca ctgagcagat tcaaggactc cctgggcaac cctacacaca
     1441 tcctgagaat cggcgagagc tacaaagaga agcagaggac aatccaggcc gccaaagagg
     1501 tggaacagag aagagccggc ggatactcta ggaagtacgc cagcaaggcc aagaatctgg
     1561 ccgacgacat ggtccgaaac accgccagag atctgctgta ctacgccgtg acacaggacg
     1621 ccatgctgat cttcgagaat ctgagcagag gcttcggccg gcagggcaag agaaccttta
     1681 tggccgagag gcagtacacc agaatggaag attggctcac agctaaactg gcctacgagg
     1741 gactgcccag caagacctac ctgtccaaaa cactggccca gtatacctcc cgcacctgca
     1801 gcaattgcgg cttcaccatc accagcgccg actacgacag agtgctggaa aagctcaaga
     1861 aaaccgccac cggctggatg accaccatca acggcaaaga gctgaaggtt gagggccaga
     1921 tcacctacta caacaggtac aagaggcaga acgtcgtgaa ggatctgagc gtggaactgg
     1981 acagactgag cgaagagagc gtgaacaacg acatcagcag ctggacaaag ggcagatcag
     2041 gcgaggctct gagcctgctg aagaagaggt ttagccacag acctgtgcaa gagaagttcg
     2101 tgtgcctgaa ctgcggcttc gagacacacg ccgatgaaca ggctgccctg aacattgcca
     2161 gaagctggct gttcctgaga agccaagagt acaagaagta ccagaccaac aagaccaccg
     2221 gcaacaccga caagagggcc tttgtggaaa cctggcagag cttctacaga aaaaagctga
     2281 aagaagtctg gaagcccgcc gtgaatgcat tgccaaagaa gaagcggaag gtcggcagtt
     2341 acccatacga tgtgcctgac tacgcttatc catacgacgt acccgattac gcctatcctt
     2401 acgacgtacc tgactacgcc taagaattcc tagagctcgc tgatcagcct cgactgtgcc
     2461 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     2521 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     2581 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     2641 gaatagcagg catgctgggg aggtaccctt ttgctggcct tttgctcaca tgtgagggcc
     2701 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     2761 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     2821 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     2881 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     2941 ccgactggcg cttctatctg attactctga gcgccatcac cagcgactat gtcgtagtgg
     3001 gtaaagctcc ctcttcggag ggagcatcag agcatcaaag ggtcttctcg aagacatgcc
     3061 gtttgcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct
     3121 cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct
     3181 cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta
     3241 cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag
     3301 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3361 cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3421 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3481 cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata
     3541 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3601 aactggaaca acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc
     3661 gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3721 caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc
     3781 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     3841 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     3901 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     3961 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4021 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4081 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4141 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4201 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4261 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4321 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4381 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4441 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4501 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4561 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4621 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4681 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4741 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4801 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtggaagcc gcggtatcat
     4861 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     4921 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     4981 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5041 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5101 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5161 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5221 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5281 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5341 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5401 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5461 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5521 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5581 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5641 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5701 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5761 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt cctgcaggca
     5821 gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt ggtcgcccgg
     5881 cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact aggggttcct
     5941 gcggcctcta gactcgaggc gttgacattg attattgact agttattaat agtaatcaat
     6001 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
     6061 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     6121 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     6181 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     6241 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     6301 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     6361 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     6421 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     6481 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     6541 cagagctctc tggctaacta ccggttctag agcgctgcca ccatgtccgg atcccctgct
     6601 gccaagaggg tcaagttgga catgcaagag atcaagagaa tcaacaagat cagaaggaga
     6661 ctggtcaagg acagcaacac aaagaaggcc ggcaagcgcg gccccatgaa aaccctgctc
     6721 gtcagagtga tgacccctga cctgagagag cggctggaaa acctgagaaa gaagcccgag
     6781 aacatccctc agcctatcag caacaccagc agggccaacc tgaacaagct gctgaccgac
     6841 tacaccgaga tgaagaaagc catcctgcac gtgtactggg aagagttcca gaaagacccc
     6901 gtgggcctga tgagcagagt tgctcagccc gctcctaaga acatcgacca gagaaagctg
     6961 atccccgtga aggacggcaa cgagagactg acctctagcg gctttgcctg cagccagtgt
     7021 tgccagcctc tgtacgtgta caagctggaa caagtgaacg acaagggcaa gccccacacc
     7081 aactacttcg gcagatgcaa cgtgtccgag cacgagaggc tgatcctgct gtctcctcac
     7141 aagcccgagg ccaacgatga gctggtcaca tacagcctgg gcaagttcgg acagagagcc
     7201 ctggacttct acagcatcca cgtgaccagg gagagcaatc accctgtgaa gc
//
