LOCUS       plasmid                 7252 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(3691..4551)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(2338..2717)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(2134..2337)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   complement(5468..5608)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(6116..6227)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(4552..4656)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(2932..3520)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(4938..5393)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(2060..2086)
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(5747..5987)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    5412..5463
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(6289..6311)
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    4888..4937
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             4751..4786
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(6319..6341)
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(2832..2859)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   complement(2776..2803)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 tgcccaggga gtccttgaat ctgctcagtg ggcatccttc agggtctgtc agggcgatca
       61 cagcagggat attctcgccc cggtcgatgc cgatcaggtt catgggcttg atgttgctgc
      121 tgtccagcac ctctcttctc tcgaaggtca gggccacaaa cagagcaggc tcgtcctgtc
      181 tggttctcct gttgtacagg gttttctcga tcactctgcc attggccagg cgcagagagc
      241 cggtttccag gctcagcaga tcgttccaga tgaactctct gccctgtctc ttgccgaagg
      301 ccagaggcag gataatcagg ttggggtcgt cgaagttgaa gttcacttcc atgggcacga
      361 tctcgccgga ctttttgttg atcacggtgt agaatctgtt agcctcgaag gcctcgggtt
      421 tgatcttctt gaaccgcagc ttgccgcctt tgaagtaatt gatgatcagg tacaggttca
      481 gtttcttgac gccgtctttc tgccaaatga aggcgcagtt gtactgcttg ctgaagccgc
      541 tgatgtccag gatgctgttc tcggcctcaa tggcgaaggg cttgcctctc agatcgccgt
      601 accacttctg cagcttcagc tcgcatctgc agaactcgtc cttgtcggcc tctttcaggc
      661 cctcgatcac aaagctggcc ttggctctca gccaatcggt cagagcggct ttagattggg
      721 cgtcctcgct ccttctttcc tcttccagct taatgtgctt gctcaggcct tccaccttct
      781 tgtcgattct ctcccaggcc tcatcgtaca ctttgcccca gtcctcgccg tgcttctttt
      841 ccaggtgcag cagcaggtcg ccgaactggt atctggcgaa cttcttgccc ttcttccggt
      901 cctcttcgct gctcaggtaa ggcagcaggg cttcttgtct cttgtagccg gccaggttct
      961 gccagaaaac cttgccatcc tctttcttct cgttgatcag cttcttcacg ttgcagacca
     1021 tgtcccacca atccacttca ttggcctgtc tttccaccag agggaagcta gggaagccct
     1081 tcagtctctg cagtggcttg gcttcgtccc tgccgatctt cagcttctgc cacagattca
     1141 ggttgaccca aatcacgatc tgggccacca cgttgttgta ggcctcgatg ccctctttgg
     1201 tgtgaggctg tggaggcagg gtgatcttag gaaaggccag gccgttagcg ctggcgatat
     1261 ccttgaggtt ggccagtctt ttctcgttct tcttgatcac tttctggtgt tccaggatga
     1321 tgtcctggta cttggtcagg aaggatgcca cggctcccat acaggcgtcg ctcagggctt
     1381 ttcccacagg tccagaggca cagctattgc cgccgatctg ttccaggggc ttcacagggt
     1441 gattgctctc cctggtcacg tggatgctgt agaagtccag ggctctctgt ccgaacttgc
     1501 ccaggctgta tgtgaccagc tcatcgttgg cctcgggctt gtgaggagac agcaggatca
     1561 gcctctcgtg ctcggacacg ttgcatctgc cgaagtagtt ggtgtggggc ttgcccttgt
     1621 cgttcacttg ttccagcttg tacacgtaca gaggctggca acactggctg caggcaaagc
     1681 cgctagaggt cagtctctcg ttgccgtcct tcacggggat cagctttctc tggtcgatgt
     1741 tcttaggagc gggctgagca actctgctca tcaggcccac ggggtctttc tggaactctt
     1801 cccagtacac gtgcaggatg gctttcttca tctcggtgta gtcggtcagc agcttgttca
     1861 ggttggccct gctggtgttg ctgataggct gagggatgtt ctcgggcttc tttctcaggt
     1921 tttccagccg ctctctcagg tcaggggtca tcactctgac gagcagggtt ttcatggggc
     1981 cgcgcttgcc ggccttcttt gtgttgctgt ccttgaccag tctccttctg atcttgttga
     2041 ttctcttgat ctcttgcatg tccaacttga ccctcttggc agcaggggat ccggacatgg
     2101 tggcagcgct ctagaaccgg tagttagcca gagagctctg cttatataga cctcccaccg
     2161 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     2221 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     2281 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     2341 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     2401 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     2461 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     2521 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     2581 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     2641 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     2701 agtcaataat caatgtcaac gcctcgagtc tagaggccgc aggaacccct agtgatggag
     2761 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     2821 cgacgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg acatgtgagc
     2881 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2941 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3001 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3061 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3121 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3181 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3241 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3301 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3361 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3421 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     3481 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     3541 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     3601 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     3661 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3721 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3781 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc ggcttccacg
     3841 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3901 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3961 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     4021 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     4081 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     4141 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     4201 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     4261 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     4321 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     4381 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     4441 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     4501 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     4561 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     4621 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     4681 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     4741 gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
     4801 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     4861 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     4921 actgagagtg caccataaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
     4981 ttgttaaatc agctcatttt ttaaccaata gaccgaaatc ggcaaaatcc cttataaatc
     5041 aaaagaatag cccgagatag agttgagtgt tgttccagtt tggaacaaga gtccactatt
     5101 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     5161 acgtgaacca tcacccaaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     5221 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5281 aaaggaaggg aagaaagcga aaggagcggg cgctaaggcg ctggcaagtg tagcggtcac
     5341 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtactatgg
     5401 ttgctttgac gtatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     5461 aggcgcccct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc
     5521 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
     5581 gtggccaact ccatcactag gggttcctgc ggccgcaaac ggcatgtctt cgagaagacc
     5641 ctttgatgct ctgatgctcc ctccgaagag ggagctttac ccactacgac atagtcgctg
     5701 gtgatggcgc tcagagtaat cagatagaag cgccagtcgg tgtttcgtcc tttccacaag
     5761 atatataaag ccaagaaatc gaaatacttt caagttacgg taagcatatg atagtccatt
     5821 ttaaaacata attttaaaac tgcaaactac ccaagaaatt attactttct acgtcacgta
     5881 ttttgtacta atatctttgt gtttacagtc aaattaattc caattatctc tctaacagcc
     5941 ttgtatcgta tatgcaaata tgaaggaatc atgggaaata ggccctcaca tgtgagcaaa
     6001 aggccagcaa aagggtacct ccccagcatg cctgctattc tcttcccaat cctccccctt
     6061 gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac aatgcgatgc
     6121 aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg tcaaggaagg
     6181 cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg aggctgatca
     6241 gcgagctcta ggaattctta ggcgtagtca ggtacgtcgt aaggataggc gtaatcgggt
     6301 acgtcgtatg gataagcgta gtcaggcaca tcgtatgggt aactgccgac cttccgcttc
     6361 ttctttggca atgcattcac ggcgggcttc cagacttctt tcagcttttt tctgtagaag
     6421 ctctgccagg tttccacaaa ggccctcttg tcggtgttgc cggtggtctt gttggtctgg
     6481 tacttcttgt actcttggct tctcaggaac agccagcttc tggcaatgtt cagggcagcc
     6541 tgttcatcgg cgtgtgtctc gaagccgcag ttcaggcaca cgaacttctc ttgcacaggt
     6601 ctgtggctaa acctcttctt cagcaggctc agagcctcgc ctgatctgcc ctttgtccag
     6661 ctgctgatgt cgttgttcac gctctcttcg ctcagtctgt ccagttccac gctcagatcc
     6721 ttcacgacgt tctgcctctt gtacctgttg tagtaggtga tctggccctc aaccttcagc
     6781 tctttgccgt tgatggtggt catccagccg gtggcggttt tcttgagctt ttccagcact
     6841 ctgtcgtagt cggcgctggt gatggtgaag ccgcaattgc tgcaggtgcg ggaggtatac
     6901 tgggccagtg ttttggacag gtaggtcttg ctgggcagtc cctcgtaggc cagtttagct
     6961 gtgagccaat cttccattct ggtgtactgc ctctcggcca taaaggttct cttgccctgc
     7021 cggccgaagc ctctgctcag attctcgaag atcagcatgg cgtcctgtgt cacggcgtag
     7081 tacagcagat ctctggcggt gtttcggacc atgtcgtcgg ccagattctt ggccttgctg
     7141 gcgtacttcc tagagtatcc gccggctctt ctctgttcca cctctttggc ggcctggatt
     7201 gtcctctgct tctctttgta gctctcgccg attctcagga tgtgtgtagg gt
//
