LOCUS       plasmid                 7386 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2167..3027
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        4001..4380
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        4381..4584
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   1110..1250
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    491..602
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        2062..2166
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      3198..3786
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      1325..1780
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             4632..4658
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        731..971
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(1255..1306)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             407..429
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    complement(1781..1830)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(1932..1967)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             377..399
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   3859..3886
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   3915..3942
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 tctgagcgtg gaactggaca gactgagcga agagagcgtg aacaacgaca tcagcagctg
       61 gacaaagggc agatcaggcg aggctctgag cctgctgaag aagaggttta gccacagacc
      121 tgtgcaagag aagttcgtgt gcctgaactg cggcttcgag acacacgccg atgaacaggc
      181 tgccctgaac attgccagaa gctggctgtt cctgagaagc caagagtaca agaagtacca
      241 gaccaacaag accaccggca acaccgacaa gagggccttt gtggaaacct ggcagagctt
      301 ctacagaaaa aagctgaaag aagtctggaa gcccgccgtg aatgcattgc caaagaagaa
      361 gcggaaggtc ggcagttacc catacgatgt gcctgactac gcttatccat acgacgtacc
      421 cgattacgcc tatccttacg acgtacctga ctacgcctaa gaattcctag agctcgctga
      481 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      541 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
      601 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
      661 ggggaggatt gggaagagaa tagcaggcat gctggggagg tacccttttg ctggcctttt
      721 gctcacatgt gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
      781 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
      841 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      901 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      961 gtggaaagga cgaaacaccg actggcgctt ctatctgatt actctgagcg ccatcaccag
     1021 cgactatgtc gtagtgggta aagctccctc ttcggaggga gcatcagagc atcaaagggt
     1081 cttctcgaag acatgccgtt tgcggccgca ggaaccccta gtgatggagt tggccactcc
     1141 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg
     1201 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat
     1261 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca
     1321 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1381 accgctacac ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc
     1441 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     1501 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt
     1561 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     1621 agtggactct tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat
     1681 ttataaggga ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa
     1741 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca
     1801 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     1861 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1921 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     1981 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     2041 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2161 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     2221 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     2281 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     2341 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     2401 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     2461 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     2521 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     2581 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     2641 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     2701 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     2761 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     2821 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2881 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2941 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     3001 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     3061 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     3121 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     3181 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     3241 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     3301 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     3361 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     3421 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     3481 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     3541 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     3601 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     3661 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     3721 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     3781 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3841 cacatgtcct gcaggcagct gcgcgctcgc tcgctcactg aggccgcccg ggcgtcgggc
     3901 gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag tggccaactc
     3961 catcactagg ggttcctgcg gcctctagac tcgaggcgtt gacattgatt attgactagt
     4021 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4081 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4141 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4201 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4261 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4321 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     4381 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
     4441 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     4501 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
     4561 tgggaggtct atataagcag agctctctgg ctaactaccg gttctagagc gctgccacca
     4621 tgtccggatc ccctgctgcc aagagggtca agttggacat gcaagagatc aagagaatca
     4681 acaagatcag aaggagactg gtcaaggaca gcaacacaaa gaaggccggc aagcgcggcc
     4741 ccatgaaaac cctgctcgtc agagtgatga cccctgacct gagagagcgg ctggaaaacc
     4801 tgagaaagaa gcccgagaac atccctcagc ctatcagcaa caccagcagg gccaacctga
     4861 acaagctgct gaccgactac accgagatga agaaagccat cctgcacgtg tactgggaag
     4921 agttccagaa agaccccgtg ggcctgatga gcagagttgc tcagcccgct cctaagaaca
     4981 tcgaccagag aaagctgatc cccgtgaagg acggcaacga gagactgacc tctagcggct
     5041 ttgcctgcag ccagtgttgc cagcctctgt acgtgtacaa gctggaacaa gtgaacgaca
     5101 agggcaagcc ccacaccaac tacttcggca gatgcaacgt gtccgagcac gagaggctga
     5161 tcctgctgtc tcctcacaag cccgaggcca acgatgagct ggtcacatac agcctgggca
     5221 agttcggaca gagagccctg gacttctaca gcatccacgt gaccagggag agcaatcacc
     5281 ctgtgaagcc cctggaacag atcggcggca atagctgtgc ctctggacct gtgggaaaag
     5341 ccctgagcga cgcctgtatg ggagccgtgg catccttcct gaccaagtac caggacatca
     5401 tcctggaaca ccagaaagtg atcaagaaga acgagaaaag actggccaac ctcaaggata
     5461 tcgccagcgc taacggcctg gcctttccta agatcaccct gcctccacag cctcacacca
     5521 aagagggcat cgaggcctac aacaacgtgg tggcccagat cgtgatttgg gtcaacctga
     5581 atctgtggca gaagctgaag atcggcaggg acgaagccaa gccactgcag agactgaagg
     5641 gcttccctag cttccctctg gtggaaagac aggccaatga agtggattgg tgggacatgg
     5701 tctgcaacgt gaagaagctg atcaacgaga agaaagagga tggcaaggtt ttctggcaga
     5761 acctggccgg ctacaagaga caagaagccc tgctgcctta cctgagcagc gaagaggacc
     5821 ggaagaaggg caagaagttc gccagatacc agttcggcga cctgctgctg cacctggaaa
     5881 agaagcacgg cgaggactgg ggcaaagtgt acgatgaggc ctgggagaga atcgacaaga
     5941 aggtggaagg cctgagcaag cacattaagc tggaagagga aagaaggagc gaggacgccc
     6001 aatctaaagc cgctctgacc gattggctga gagccaaggc cagctttgtg atcgagggcc
     6061 tgaaagaggc cgacaaggac gagttctgca gatgcgagct gaagctgcag aagtggtacg
     6121 gcgatctgag aggcaagccc ttcgccattg aggccgagaa cagcatcctg gacatcagcg
     6181 gcttcagcaa gcagtacaac tgcgccttca tttggcagaa agacggcgtc aagaaactga
     6241 acctgtacct gatcatcaat tacttcaaag gcggcaagct gcggttcaag aagatcaaac
     6301 ccgaggcctt cgaggctaac agattctaca ccgtgatcaa caaaaagtcc ggcgagatcg
     6361 tgcccatgga agtgaacttc aacttcgacg accccaacct gattatcctg cctctggcct
     6421 tcggcaagag acagggcaga gagttcatct ggaacgatct gctgagcctg gaaaccggct
     6481 ctctgcgcct ggccaatggc agagtgatcg agaaaaccct gtacaacagg agaaccagac
     6541 aggacgagcc tgctctgttt gtggccctga ccttcgagag aagagaggtg ctggacagca
     6601 gcaacatcaa gcccatgaac ctgatcggca tcgaccgggg cgagaatatc cctgctgtga
     6661 tcgccctgac agaccctgaa ggatgcccac tgagcagatt caaggactcc ctgggcaacc
     6721 ctacacacat cctgagaatc ggcgagagct acaaagagaa gcagaggaca atccaggccg
     6781 ccaaagaggt ggaacagaga agagccggcg gatactctag gaagtacgcc agcaaggcca
     6841 agaatctggc cgacgacatg gtccgaaaca ccgccagaga tctgctgtac tacgccgtga
     6901 cacaggacgc catgctgatc ttcgagaatc tgagcagagg cttcggccgg cagggcaaga
     6961 gaacctttat ggccgagagg cagtacacca gaatggaaga ttggctcaca gctaaactgg
     7021 cctacgaggg actgcccagc aagacctacc tgtccaaaac actggcccag tatacctccc
     7081 gcacctgcag caattgcggc ttcaccatca ccagcgccga ctacgacaga gtgctggaaa
     7141 agctcaagaa aaccgccacc ggctggatga ccaccatcaa cggcaaagag ctgaaggttg
     7201 agggccagat cacctactac aacaggtaca agaggcagaa cgtcgtgaag gatctgagcg
     7261 tggaactgga cagactgagc gaagagagcg tgaacaacga catcagcagc tggacaaagg
     7321 gcagatcagg cgaggctctg agcctgctga agaagaggtt tagccacaga cctgtgcaag
     7381 agaagt
//
