LOCUS       plasmid                 7252 bp    DNA     circular SYN 22-JAN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(838..1698)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     enhancer        complement(6737..7116)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(6533..6736)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   complement(2615..2755)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(3263..3374)
                     /note="pLannotate"
                     /label="bGH poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(1699..1803)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     rep_origin      complement(79..667)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      complement(2085..2540)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     CDS             complement(6459..6485)
                     /note="pLannotate"
                     /label="c-myc NLS"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     promoter        complement(2894..3134)
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     repeat_region   7140..7231
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="65.2"
                     /fragment="True"
                     /other="repeat_region"
     misc_feature    2559..2610
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3436..3458)
                     /note="pLannotate"
                     /label="HA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="85.2"
                     /fragment="True"
                     /other="CDS"
     misc_feature    2035..2084
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1898..1933
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3466..3488)
                     /note="pLannotate"
                     /label="3xHA (fragment)"
                     /database="snapgene"
                     /identity="95.7"
                     /match_length="25.6"
                     /fragment="True"
                     /other="CDS"
     repeat_region   complement(join(7231..7252,1..6))
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 gcgcagctgc ctgcaggaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
       61 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      181 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
      241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
      301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
      421 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
      481 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
      541 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      601 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      661 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
      721 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
      781 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
      841 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
      901 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
      961 tgctgcaatg ataccgcggc ttccacgctc accggctcca gatttatcag caataaacca
     1021 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     1081 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     1141 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     1201 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     1261 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     1321 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     1381 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     1441 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     1501 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     1561 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     1621 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     1681 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     1741 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     1801 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     1861 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     1921 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     1981 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     2041 taactatgcg gcatcagagc agattgtact gagagtgcac cataaaattg taaacgttaa
     2101 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaatagac
     2161 cgaaatcggc aaaatccctt ataaatcaaa agaatagccc gagatagagt tgagtgttgt
     2221 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     2281 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg
     2341 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     2401 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     2461 taaggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     2521 tgcgccgcta cagggcgcgt actatggttg ctttgacgta tgcggtgtga aataccgcac
     2581 agatgcgtaa ggagaaaata ccgcatcagg cgcccctgca ggcagctgcg cgctcgctcg
     2641 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
     2701 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc
     2761 cgcaaacggc atgtcttcga gaagaccctt tgatgctctg atgctccctc cgaagaggga
     2821 gctttaccca ctacgacata gtcgctggtg atggcgctca gagtaatcag atagaagcgc
     2881 cagtcggtgt ttcgtccttt ccacaagata tataaagcca agaaatcgaa atactttcaa
     2941 gttacggtaa gcatatgata gtccatttta aaacataatt ttaaaactgc aaactaccca
     3001 agaaattatt actttctacg tcacgtattt tgtactaata tctttgtgtt tacagtcaaa
     3061 ttaattccaa ttatctctct aacagccttg tatcgtatat gcaaatatga aggaatcatg
     3121 ggaaataggc cctcacatgt gagcaaaagg ccagcaaaag ggtacctccc cagcatgcct
     3181 gctattctct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     3241 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     3301 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     3361 actagaaggc acagtcgagg ctgatcagcg agctctagga attcttaggc gtagtcaggt
     3421 acgtcgtaag gataggcgta atcgggtacg tcgtatggat aagcgtagtc aggcacatcg
     3481 tatgggtaac tgccgacctt ccgcttcttc tttggcaatg cattcacggc gggcttccag
     3541 acttctttca gcttttttct gtagaagctc tgccaggttt ccacaaaggc cctcttgtcg
     3601 gtgttgccgg tggtcttgtt ggtctggtac ttcttgtact cttggcttct caggaacagc
     3661 cagcttctgg caatgttcag ggcagcctgt tcatcggcgt gtgtctcgaa gccgcagttc
     3721 aggcacacga acttctcttg cacaggtctg tggctaaacc tcttcttcag caggctcaga
     3781 gcctcgcctg atctgccctt tgtccagctg ctgatgtcgt tgttcacgct ctcttcgctc
     3841 agtctgtcca gttccacgct cagatccttc acgacgttct gcctcttgta cctgttgtag
     3901 taggtgatct ggccctcaac cttcagctct ttgccgttga tggtggtcat ccagccggtg
     3961 gcggttttct tgagcttttc cagcactctg tcgtagtcgg cgctggtgat ggtgaagccg
     4021 caattgctgc aggtgcggga ggtatactgg gccagtgttt tggacaggta ggtcttgctg
     4081 ggcagtccct cgtaggccag tttagctgtg agccaatctt ccattctggt gtactgcctc
     4141 tcggccataa aggttctctt gccctgccgg ccgaagcctc tgctcagatt ctcgaagatc
     4201 agcatggcgt cctgtgtcac ggcgtagtac agcagatctc tggcggtgtt tcggaccatg
     4261 tcgtcggcca gattcttggc cttgctggcg tacttcctag agtatccgcc ggctcttctc
     4321 tgttccacct ctttggcggc ctggattgtc ctctgcttct ctttgtagct ctcgccgatt
     4381 ctcaggatgt gtgtagggtt gcccagggag tccttgaatc tgctcagtgg gcatccttca
     4441 gggtctgtca gggcgatcac agcagggata ttctcgcccc ggtcgatgcc gatcaggttc
     4501 atgggcttga tgttgctgct gtccagcacc tctcttctct cgaaggtcag ggccacaaac
     4561 agagcaggct cgtcctgtct ggttctcctg ttgtacaggg ttttctcgat cactctgcca
     4621 ttggccaggc gcagagagcc ggtttccagg ctcagcagat cgttccagat gaactctctg
     4681 ccctgtctct tgccgaaggc cagaggcagg ataatcaggt tggggtcgtc gaagttgaag
     4741 ttcacttcca tgggcacgat ctcgccggac tttttgttga tcacggtgta gaatctgtta
     4801 gcctcgaagg cctcgggttt gatcttcttg aaccgcagct tgccgccttt gaagtaattg
     4861 atgatcaggt acaggttcag tttcttgacg ccgtctttct gccaaatgaa ggcgcagttg
     4921 tactgcttgc tgaagccgct gatgtccagg atgctgttct cggcctcaat ggcgaagggc
     4981 ttgcctctca gatcgccgta ccacttctgc agcttcagct cgcatctgca gaactcgtcc
     5041 ttgtcggcct ctttcaggcc ctcgatcaca aagctggcct tggctctcag ccaatcggtc
     5101 agagcggctt tagattgggc gtcctcgctc cttctttcct cttccagctt aatgtgcttg
     5161 ctcaggcctt ccaccttctt gtcgattctc tcccaggcct catcgtacac tttgccccag
     5221 tcctcgccgt gcttcttttc caggtgcagc agcaggtcgc cgaactggta tctggcgaac
     5281 ttcttgccct tcttccggtc ctcttcgctg ctcaggtaag gcagcagggc ttcttgtctc
     5341 ttgtagccgg ccaggttctg ccagaaaacc ttgccatcct ctttcttctc gttgatcagc
     5401 ttcttcacgt tgcagaccat gtcccaccaa tccacttcat tggcctgtct ttccaccaga
     5461 gggaagctag ggaagccctt cagtctctgc agtggcttgg cttcgtccct gccgatcttc
     5521 agcttctgcc acagattcag gttgacccaa atcacgatct gggccaccac gttgttgtag
     5581 gcctcgatgc cctctttggt gtgaggctgt ggaggcaggg tgatcttagg aaaggccagg
     5641 ccgttagcgc tggcgatatc cttgaggttg gccagtcttt tctcgttctt cttgatcact
     5701 ttctggtgtt ccaggatgat gtcctggtac ttggtcagga aggatgccac ggctcccata
     5761 caggcgtcgc tcagggcttt tcccacaggt ccagaggcac agctattgcc gccgatctgt
     5821 tccaggggct tcacagggtg attgctctcc ctggtcacgt ggatgctgta gaagtccagg
     5881 gctctctgtc cgaacttgcc caggctgtat gtgaccagct catcgttggc ctcgggcttg
     5941 tgaggagaca gcaggatcag cctctcgtgc tcggacacgt tgcatctgcc gaagtagttg
     6001 gtgtggggct tgcccttgtc gttcacttgt tccagcttgt acacgtacag aggctggcaa
     6061 cactggctgc aggcaaagcc gctagaggtc agtctctcgt tgccgtcctt cacggggatc
     6121 agctttctct ggtcgatgtt cttaggagcg ggctgagcaa ctctgctcat caggcccacg
     6181 gggtctttct ggaactcttc ccagtacacg tgcaggatgg ctttcttcat ctcggtgtag
     6241 tcggtcagca gcttgttcag gttggccctg ctggtgttgc tgataggctg agggatgttc
     6301 tcgggcttct ttctcaggtt ttccagccgc tctctcaggt caggggtcat cactctgacg
     6361 agcagggttt tcatggggcc gcgcttgccg gccttctttg tgttgctgtc cttgaccagt
     6421 ctccttctga tcttgttgat tctcttgatc tcttgcatgt ccaacttgac cctcttggca
     6481 gcaggggatc cggacatggt ggcagcgctc tagaaccggt agttagccag agagctctgc
     6541 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     6601 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     6661 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     6721 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     6781 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     6841 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     6901 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     6961 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     7021 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     7081 ttgattacta ttaataacta gtcaataatc aatgtcaacg cctcgagtct agaggccgca
     7141 ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc
     7201 cgggcgacca aaggtcgccc gacgcccggg cggcctcagt gagcgagcga gc
//
